PSMI Micro-Incubator

PSMI Micro-Incubator

Description

DESCRIPTION

The PSMI Micro-Incubator System provides a complete solution to the challenge of electrophysiological study of synaptic connections at physiological temperatures. Low noise whole cell patch recording, with or without water immersion of the microscope objective in the recording medium, are possible with the PSMI.

This PSMI Micro-Incubator is a shallow, annular assembly which surrounds the central slice holding dish. Room temperature perfusant is delivered to the incubator which in turn delivers it to the dish at a precisely controlled temperature you select. The perfusant is removed (via user supplied suction) by an included, height adjustable aspirator (LU-ASP). Two independent perfusant channels flow through the PSMI into the chamber via replaceable plastic tubing (of low volumes <100 µl) to allow rapid switching of media via an upstream valve for pharmacological studies.
A separate inlet provides temperature controlled gas delivery across the dish.

The TC-202A provides temperature control (~5° below ambient to 50°C) using the Micro-Incubator warming/cooling plate as the control point. Lower temperatures (approximately 10°C below ambient) may be obtained by using an optional cooling tube. The cover slip dish (PS-CSD) consists of two 22 mm diameter cover slips held in a circular frame. The slice sits on the top cover slip with the immersion objective reaching the fluid surface. The bottom cover slip prevents condensation for optimal illumination and viewing. To enable placement of the patch electrode, entry angles as small as 15° can be achieved for slice access.

Specification

Chamber Type PS-CSD Cover Slip Dish
Temperature Stability ±0.2°C with TC-202A, at 37°C with 1 ml/min perfusion
Temperature Gradient Across chamber: 2°C with 1.5 ml media volume, 1 ml/min
Temperature Range 5-10°C below ambient to 50°C
Built In Temp. Sensor Thermistor type: 100 kΩ at 25°C
Peltier Device Current rating: 6 A max
Media Perfusion Rates Up to 3.0 ml/min
Dimensions, H x Dia 17 x 152 mm (0.67 x 6.0 in), overall
Weight 0.5 kg (17.9 oz)

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Product Information

PSMI Micro-Incubator

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