Chamber Slide Micro-Incubator (CSMI)

Chamber Slide Micro-Incubator (CSMI)



The Chamber Slide Micro-Incubator (CSMI) is a versatile microscope stage-incubator for cell/tissue culture work. It is designed to accommodate disposable rectangular Chamber Slides™ and Chambered Coverglass from the Nunc Lab-Tek® series, and the Falcon® Culture slide series from Beckton Dickinson.

The CSMI, in combination with a matching low noise TC-202A temperature controller, facilitates long term maintenance of tissue or cell cultures on a microscope stage. This allows optical monitoring of dyes, micro-injection, electrical recording, and micro-manipulation for many hours or even days.

The CSMI not only facilitates the precise regulation and manipulation of bath temperature, but also supports multi-channel perfusion (up to 4 lines), gas atmosphere maintenance, and CO2 superfusion for pH control.

The CSMI is based on a successful design of the Medical Systems PDMI-2 Micro-Incubator for 35 mm Petri dishes. The CSMI utilizes Peltier technology to regulate temperature over a wide range of temperatures, both above and below ambient levels. Lower temperatures (approximately 10°C below ambient) may be obtained by using an optional cooling tube.

The CSMI fits on the stage of inverted microscopes from major microscope manufacturers including Zeiss, Nikon, Olympus and Leica. Harvard Apparatus offers custom-made rectangular adapters for these microscopes.


Temperature Range ~5° to 10°C below ambient to 50°C
Disposable Chambers Accommodated Nalge Nunc chambered slides & cover slips
Becton Dickinson Falcon chambered slides
Microscopes Accommodated Zeiss Axiovert with attachable mechanical stage
Leica DAS Microscope DMIL and DMIRB/E with attached mechanical stage
Nikon Diaphot
Olympus IX50/70 or IMT-2 fixed stage
Peltier TED Current Rating 6 A DC max
Built In Plate Thermistor 100 kΩ at 25°C
Perfusion Inlet Lines 26 AWG Teflon capillary up to 4 lines can be installed simultaneously
Perfusion Rate 3 ml/min nominal total
Perfusate Outlet LU-ASP aspirator
Gas Port 1/16 in. barb
Gas Superfusion Rate 0.5 to 2.0 L/min
Weight 17.9 oz (0.5 kg)
Overall Dimensions 6.5 x 4 x 1 in (16.5 x 10.2 x 2.5 cm)

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Product Information

Chamber Slide Micro-Incubator




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