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Flyion
GmbH have developed a revolutionary system for obtaining automatic
and reliable whole cell recordings from ion channels expressed in
mammalian cells. Using "Flip the Tip" technology,
a specially fabricated glass microelectrode is filled with a cell
suspension and giga-ohm seals are made between cells flushed
towards the tip. By applying suction from the outside of the
tip, a stable whole cell recording can be achieved with extremely
low access resistance (approx. 3Mohm). Alternatively, the
system is also capable of making perforated patch
recordings. The Flyscreen 8500 robot not only carries out
all the above steps automatically, but can then go on to apply
compounds of interest to the cells, running predefined voltage
clamp protocols and carry out initial analysis of the data
obtained. The only user intervention required relates to the
refilling of solutions and replacement of FlipTip and compound
racks. Primarily developed for pharmaceutical companies
wanting to carry out screening of drug candidates for effects on
ion channels (e.g HERG), the Flyscreen 8500 is also available to academic
institutions.
Flyion
FLYSCREEN 8500 Patch Clamp Robot |
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The
Flyscreen 8500 patch clamp robot
is a fully automated patch clamp system for
electrophysiology laboratories or shared facilities within
the academic environment. The 3 or 6 channel robot can
be supplied with HEKA EPC10-2 Patch Clamp Amplifiers,
Personal Computer with two TFT screens and Flyscreen
Suite/Patchmaster software packages.
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Reference:
Lepple-Wienhuse,
A., Ferlinz, K., Seeger, A. & Schafer, A. (2003)
Flip the Tip: An Automated, High Quality, Cost-Effective
Patch Clamp Screen. Receptors and Channels 9:
13-17.
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